rac1 (Cytoskeleton Inc)
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Cytoskeleton Inc
rac1
Rac1, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rac1/Anti-Rac1+mouse+Mab/pmc13161276-452-43-47
Average 94 stars, based on 239 article reviews
Rac1, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rac1/Anti-Rac1+mouse+Mab/pmc13161276-452-43-47
Average 94 stars, based on 239 article reviews
rac1 - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Endocrine therapy reprogramming of breast cancer facilitates metastatic escape via upregulation of P-Rex1/Rac1 signalling"
Article Title: Endocrine therapy reprogramming of breast cancer facilitates metastatic escape via upregulation of P-Rex1/Rac1 signalling
Journal: Nature Communications
doi: 10.1038/s41467-026-70683-x
Figure Legend Snippet: A Gene set enrichment analysis on Fast-growing compared to parental cells ( n = 288 genes) and Endocrine Tolerant resistant cells compared to parental cells ( n = 288 genes) using Curated.MSigDB. B / C Steiner Forest signalling network analysis in upregulated genes ( n = 288) of B Fast-growing and C Endocrine Tolerant cells. Node size indicates number of attached edges, with nodes with >8 in bold. Node colours: Brown=genes, light blue=phenotype, dark blue=protein complex, pink=protein family. D Multiplexed analysis of EGFR phosphorylation in: basal cells, 30 min 100 ng/mL EGF, 30 min 100 nM 17 β-estradiol, or 30 min serum stimulation. Analysed by two-way ANOVA with Bonferroni’s multiple comparison test from n = 3 biological replicates, error bars=SD. E Western blot of EGFR, pEGFR (Y1045) and β-actin in Fast and Endocrine Tolerant cells after 100 ng/mL EGF treatment for indicated times, representative of 3 biological replicates. F Multiplexed analysis of HER2 phosphorylation following treatment described in D . Analysed by two-way ANOVA with Bonferroni’s multiple comparison test. G Western blot of HER2, pHER2 (Y1221/1222) and β-actin in Fast and Endocrine Tolerant cells after 100 ng/mL EGF stimulation for indicated times, representative of 3 biological replicates. (H) PREX1 transcripts from scRNAseq analysis of MCF-7 cells treated for 48 hours with fulvestrant ( n = 340 cells), and in Fast-growing ( n = 578 cells) and Endocrine Tolerant ( n = 659 cells). I Western blot of P-Rex1, Rac1, Pak2 and β-actin in Fast and Endocrine Tolerant cells, representative of 3 biological replicates. J Densitometry of P-Rex1 normalised to β-actin from n = 3 biological replicates. Analysed by two-sided t-test, error bars=SD. K Western blot of P-Rex1, ER and GAPDH in short-term fulvestrant and estrogen treated MCF-7 cells. Representative of 5 replicates. L Densitometry of P-Rex1 normalised to β-actin from n = 5 biological replicates, analysed by one-way ANOVA with Tukey’s multiple comparisons, error bars=SEM. M / N Multiplexed analysis of M pIGF1R and N pIR phosphorylation following treatment described in D . Analysed by two-way ANOVA with Bonferroni’s multiple comparisons. O Western blot of pIR/pIGF1R and β-actin in Fast and Endocrine Tolerant cells after 30 mins insulin. Representative of 3 replicates. P Western blot for P-Rex1 and GAPDH in Fast and Endocrine Tolerant cells expressing non-targeting (NT) and PREX1 shRNA, representative of n = 2 biological replicates. Q Quantitation of transwell migration assay of Fast and Endocrine Tolerant cells that express NT and PREX1 shRNA. Analysed by unpaired two-sided t-test from n = 3 biological replicates, error bars=SEM. R / S Correlation between PREX1 and EGFR in the (R) TCGA (RNAseq RSEM; n = 812 cancers) and S Metastatic Breast Cancer Project (RNAseq RSEM; n = 67 cancers). r=Spearman’s correlation. Orange line is least squares fit. Source data provided in Source Data file.
Techniques Used: Phospho-proteomics, Comparison, Western Blot, Expressing, shRNA, Quantitation Assay, Transwell Migration Assay, RNA sequencing
Figure Legend Snippet: A Schematic of spontaneous tumour development and dissemination in the MMTV-PyMT model. Expression of Rac1 (A_51_P513254), Prex1 (A_51_P348372) and Pak2 (A_51_P172323) in stages of MMTV-PyMT development ( GSE43566 ) of adenoma ( n = 5), carcinoma ( n = 6), dissemination ( n = 6) and metastases ( n = 5). Data analysed by one-way ANOVA with Tukey’s multiple comparisons. B Schematic of derivation of MMTV-PyMT Rac1-FRET biosensor mice with biosensor in active (blue) and inactive (red) conformations, and derivation of a chronic tamoxifen biosensor cell line. C MMTV-PyMT Rac1-FRET parental (PyMT) and chronic tamoxifen (PyMT-Tam) cell lines western blotted for ER, P-Rex1, Pak2 and Rac1. β-actin loading control is shown for each western blot. Representative of three replicates. D Densitometry of P-Rex1 expression normalised to β-actin from triplicate biological replicates, analysed by two-sided t-test, and error bars represent SEM. E Representative image of Rac1-FRET activity in MMTV-PyMT cells chronically treated with tamoxifen with matched parental control. Scale bar = 100 µm. F Quantitation of Rac1-FRET activity of cells in E . Triplicate biological replicates analysed by two-sided t-test; error bars represent SEM. G Schematic of MMTV-PyMT Rac1-FRET biosensor mouse with titanium optical imaging window implanted over a mammary tumour. H Tumour growth of control ( n = 11) and tamoxifen ( n = 9) treated MMTV-PyMT Rac1-FRET mice. Data analysed by two-way ANOVA with a mixed effect model. Error bars represent SEM. I Image of Rac1-FRET activity in control and tamoxifen treated mammary tumours, representative of three biological replicates. Scale bar = 50 µm. Source data are provided as a Source Data file.
Techniques Used: Expressing, Western Blot, Control, Activity Assay, Quantitation Assay, Optical Imaging
Figure Legend Snippet: A The Rac1 pathway can be targeted by small molecule inhibitors; NSC23766, 1A-116, R-ketorolac. B / C Endocrine Tolerant and Fast-growing cells were treated with DMSO, 100 µM R-ketorolac, 25 µM NSC23766 and 6.25 µM 1A-116 for 1 week, and colony formation detected with 10% Diff Quik Stain 2. Colony formation (% colony area) ( n = 5 biological replicates) was quantitated using Image J. Data analysed by one-way ANOVA with multiple comparisons. Error bars are SEM. D / E Wound closure in Endocrine Tolerant and Fast-growing cells treated with DMSO, 100 µM R-ketorolac, 25 µM NSC23766 and 6.25 µM 1A-116 for 24 hours. Data ( n = 3 biological replicates) analysed by two-way repeated measure ANOVA for each treatment versus vehicle. Error bars are SEM, or smaller than the symbol. F Schematic of tamoxifen resistant MMTV-PyMT:Rac1-FRET biosensor mouse with titanium window implanted over mammary tumour to monitor Rac1-FRET activity after treatment with NSC23766 (4 mg/kg; intraperitoneal injection) or R-ketorolac (1 mg/kg; oral gavage). G Representative image of Rac1-FRET activity in tamoxifen resistant (TamR) MMTV-PyMT tumours treated with a single injection 4 mg/kg NSC23766, and single cell based temporal quantitation of Rac1-FRET activity of tumour. Data ( n = 4 mice measured at 0, 2, 24 h; n = 2 at 6 h) analysed by one-way ANOVA with Dunnett’s multiple comparison test, error bars are SEM. H Representative image of Rac1-FRET activity in tamoxifen resistant (TamR) MMTV-PyMT tumours treated with 1 mg/kg R-ketorolac and imaged for up to 96 hours, and single cell based temporal quantitation of Rac1-FRET activity of tumour. Data ( n = 4 mice measured at 0, 6, 24, 48, 72, 96 h; n = 2 at 2 h) analysed by one-way ANOVA with Dunnett’s multiple comparison test, error bars are SEM. Source data are provided as a Source Data file.
Techniques Used: Diff-Quik, Staining, Activity Assay, Injection, Single Cell, Quantitation Assay, Comparison
Related Articles
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